Functional
Expression of Guinea Pig Growth Hormone Receptor
and Its Mutants in Mammalian Cells
LIAO Zhi-Yong, ZHANG Xin-Na, JING Nai-He, ZHU Shang-Quan*
( Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China )
Abstract The cDNA of guinea pig (Cavia
porcellus) growth hormone receptor (gpGHR) was cloned using RT-PCR in our
laboratory. By sequence alignment, substitutions of amino acids conserved in
other mammalian GHRs were found. For example, histidine-168 and tyrosine-332
equivalent to positions 170 and 333 in other mammalian GHRs, which were
considered to be necessary for the dimerization of GHR and the specific
GH-stimulated functions respectively, were replaced by tyrosine and serine in
gpGHR. Here, we report the functional expression of gpGHR and its mutants,
gpGHRY168H and gpGHRS332Y, in COS-7 cells and/or Chinese hamster ovary (CHO)
cells. It was shown that the COS-7 cells transfected with pcDNA3-gpGHR
possessed high affinity to bovine GH [K
Key words guinea pig; growth hormone receptor; mutagenesis; lipogenesis; protein synthesis
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